By Yonggang Ke, Pengfei Wang
This distinct quantity offers a complete technical assessment of DNA nanotechnology with an emphasis on 3D DNA nanostructure layout and purposes. assurance spans from easy layout rules for DNA and RNA nanostructures to their state-of-the-art functions in a number of fields, with the booklet divided into simple DNA and RNA nanostructure layout recommendations in addition to purposes using DNA nanostructures, together with yet now not restricted to nanomedicine, bioimaging, biosensing, nanoplasmonics, nanoelectronics, nanofabrication, crystallography, biophysics, and analytical chemistry. Written for the hugely profitable Methods in Molecular Biology sequence, chapters comprise introductions to their respective themes, lists of the required fabrics and reagents, step by step, conveniently reproducible laboratory protocols, and pointers on troubleshooting and averting identified pitfalls.
Comprehensive and authoritative, 3D DNA Nanostructure: equipment and Protocols offers the main updated educational type overviews and technical variety protocols to profit researchers in a large choice of areas.
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Extra resources for 3D DNA Nanostructure: Methods and Protocols
A) Schematic of a DNA brick, where four 8-nt domains are present. (b) Strand and LEGO model of two interacting DNA bricks, where each domain is represented by a peg or hole. (c) Layers of a cuboid structure. Figure adapted from ref.  43 Complex DNA Brick Assembly angle is present between two interacting bricks (Fig. 1b). Such architecture results in X-bricks interacting only with Y-bricks and vise versa, where X and Y indicate the orientation of the phosphodiester crossover (Fig. 1c). Thus, alternating layers of X- and Y-bricks rotated 270° clockwise in orientation are present within a structure (Fig.
4. Agarose gel electrophoresis. 75 % (g/mL) agarose gel. Gel casting: For dissolving of agarose, use microwave to heat the solution (60 mL) to facilitate the agarose melt completely in the 1× TAE buffer. Stop and shake frequently to prevent bumping. For gel casting, add 2–5 μL EB staining solution to the agarose solution (60 mL) while it cools down to about 45 °C then cast the gel into horizontal gel box. Insert the combs and wait for 30 min. Sample loading and electrophoresis: Add 10× Non-denaturing dye (1:10) to the annealed DNA structures to prepare the loading samples.
The distance between adjacent axial helices is 21 bps and the inter-thread distance is 42 bps. The design principles of creating gridiron units allow scaffold strands to travel in multiple directions, which represent an important departure from certain aspects of the previous DNA origami methods. Traditional Holliday junctions do not naturally adopt conformations that would allow them to be connected in such a way, and it was surprising to discover that these motifs could (within a larger network of crossovers) endure a 150° rotation of one of the arms while simultaneously maintaining their integrity.